82 FR 22553 - Government-Owned Invention; Availability for Licensing

DEPARTMENT OF HEALTH AND HUMAN SERVICES
National Institutes of Health

Federal Register Volume 82, Issue 93 (May 16, 2017)

Page Range22553-22553
FR Document2017-09791

The inventions listed below are owned by an agency of the U.S. Government.

Federal Register, Volume 82 Issue 93 (Tuesday, May 16, 2017)
[Federal Register Volume 82, Number 93 (Tuesday, May 16, 2017)]
[Notices]
[Page 22553]
From the Federal Register Online  [www.thefederalregister.org]
[FR Doc No: 2017-09791]


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DEPARTMENT OF HEALTH AND HUMAN SERVICES

National Institutes of Health


Government-Owned Invention; Availability for Licensing

AGENCY: National Institutes of Health, HHS.

ACTION: Notice.

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SUMMARY: The inventions listed below are owned by an agency of the U.S. 
Government.

FOR FURTHER INFORMATION CONTACT: Licensing information may be obtained 
by emailing the indicated licensing contact at the National Heart, 
Lung, and Blood, Office of Technology Transfer and Development Office 
of Technology Transfer, 31 Center Drive Room 4A29, MSC 2479, Bethesda, 
MD 20892-2479; telephone: 301-402-5579. A signed Confidential 
Disclosure Agreement may be required to receive any unpublished 
information.

SUPPLEMENTARY INFORMATION: The following inventions are available for 
licensing in accordance with 35 U.S.C. 209 and 37 CFR part 404 to 
achieve expeditious commercialization of results of federally-funded 
research and development. Technology description follows.

Efficient mRNA-Based Genetic Engineering of Human NK Cells With High-
Affinity CD16 and CCR7

    Description of Technology: A highly efficient method to genetically 
modify natural killer (NK) cells to induce expression of high affinity 
CD16 (HA-CD16) through mRNA electroporation, to potentiate NK cell-
mediated antibody-dependent cellular cytotoxicity (ADCC). ADCC is 
mediated by CD16\+\ NK cells following adoptive NK cell transfer, but 
most humans express CD16 which has a relatively low affinity for IgG1 
antibodies. However, a single nucleotide polymorphism (SNP rs396991) in 
the CD16 gene, resulting in an amino acid substitution at position 158 
(F158V), is associated with substantially higher affinity and superior 
NK cell-mediated ADCC than those with the 158F genotype. This HA-CD16-
158V polymorphism has also been linked to enhanced ADCC capacity in 
vivo. The nearly 100% efficiency of our method resulted in: (a) 
Sustained surface expression of transgenes at high levels for up to 4 
days without compromising NK cell cytotoxicity and viability; and (b) 
augmented ADCC against Daratumumab coated multiple myeloma cells by ex 
vivo expanded NK cells electroporated with mRNA coding for HA-CD16. 
This system is GMP compliant and has been used previously in FDA 
approved clinical trials.
    Potential Commercial Applications: Infusion of a large number of 
highly cytotoxic autologous ex vivo expanded NK cells expressing high-
affinity CD16 into patients, to induce a more profound anti-malignancy 
response to specific monoclonal antibodies, including: multiple myeloma 
(Daratumumab); lymphoma (Rituximab); breast cancer (Trastuzumab); and 
colon cancer (Cetuximab).
    Development Stage: Early-stage; In vitro data available.
    Inventors: Richard W. Childs and Mattias Carlsten (NHLBI).
    Publications:
    (1) Carlsten M, Levy E, Karambelkar A, Li L, Reger R, Berg M, 
Peshwa MV and Childs RW (2016) Efficient mRNA-Based Genetic Engineering 
of Human NK Cells with High-Affinity CD16 and CCR7 Augments Rituximab-
Induced ADCC against Lymphoma and Targets NK Cell Migration toward the 
Lymph Node-Associated Chemokine CCL19. Front. Immunol. 7:105. doi: 
10.3389/fimmu.2016.00105.
    (2) Carlsten M and Childs RW (2015) Genetic manipulation of NK 
cells for cancer immunotherapy: techniques and clinical implications. 
Front. Immunol. 6:266. doi: 10.3389/fimmu.2015.00266.
    Intellectual Property: NIH Reference No. E-036-2015/0,1--US 
Application No. 62/079,975, filed 14 Nov 2014; and PCT Application No. 
PCT/US2015/060646, filed 13 Nov 2015.
    Licensing Contact: Cristina Thalhammer-Reyero, Ph.D., M.B.A.; 301-
435-4507; [email protected].

    Dated: May 4, 2017.
Cristina Thalhammer-Reyero,
Senior Licensing and Patenting Manager, Office of Technology Transfer 
and Development, National Heart, Lung, and Blood Institute.
[FR Doc. 2017-09791 Filed 5-15-17; 8:45 am]
BILLING CODE 4140-01-P


Current View
CategoryRegulatory Information
CollectionFederal Register
sudoc ClassAE 2.7:
GS 4.107:
AE 2.106:
PublisherOffice of the Federal Register, National Archives and Records Administration
SectionNotices
ActionNotice.
ContactLicensing information may be obtained by emailing the indicated licensing contact at the National Heart, Lung, and Blood, Office of Technology Transfer and Development Office of Technology Transfer, 31 Center Drive Room 4A29, MSC 2479, Bethesda, MD 20892-2479; telephone: 301-402-5579. A signed Confidential Disclosure Agreement may be required to receive any unpublished information.
FR Citation82 FR 22553 

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